ISO INTERNATIONAL STANDARD 20638 Firstedition 2015-11-01 InfantformulaDetermination of nucleotides by liquid chromatography Formules infantiles-Determinationde la teneur en nucleotides par chromatographieliquide Referencenumber IS020638:2015(E) LSO @IS02015 IS020638:2015(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2015,Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8. CP 401 CH-1214 Vernier, Geneva, Switzerland Tel.+4122 749 0111 Fax +4122749 0947 [email protected] www.iso.org @ IS0 2015 - All rights reserved IS020638:2015(E) Contents Page Foreword .iv 1 Scope ..1 2 Terms and definitions ..1 3 Principle ..1 4 Reagents and materials .1 5 Apparatus .4 6 Sample preparation .4 7 Procedure .5 7.1 Extraction 5 7.2 Chromatography .5 8 Calculations. Results ..8 9 AnnexA (informative)Examples ofchromatograms AnnexB(informative)Precisiondata ..10 Bibliography .14 isnto Sandardzann rights reserved ii
ISO 20638 2015 Infant formula — Determination of nucleotides by liquid chromatography