ICS07.100.01 CCSC50 团体标准 T/CI011—2021 细菌生物被膜的培养、观测和耐药性评测 技术指南 TechnicalGuidelinesonBiofilmCultivation,Examinationand ResistanceEvaluation 2021-8-5发布 2021-8-5实施 中国国际科技促进会发布 全国团体标准信息平台 目录 1.适用范围..................................................................................................................1 2.术语和定义..............................................................................................................1 2.1生物被膜：.............................................................................................................1 2.2交叉感染：.............................................................................................................1 2.3ColonyFormingUnit(CFU):..................................................................................1 2.4群体感应：.............................................................................................................1 2.5SEM..........................................................................................................................1 3.细菌生物被膜培养与观测方法归类表..................................................................2 4.细菌生物被膜培养方法与技术标准......................................................................4 4.1体外静态生物被膜培养.........................................................................................4 4.1.1孔板培养法..........................................................................................................5 4.1.2玻璃珠培养法.......................................................................................................6 4.1.3玻片培养法..........................................................................................................8 4.1.4Peg-lid培养法.....................................................................................................9 4.2体外动态生物被膜培养.......................................................................................11 4.2.1管式培养法（tubing-biofilm）........................................................................11 4.2.2池式培养法（flow-cellbiofilm）....................................................................14 5.细菌生物被膜定量与观测技术与标准................................................................16 5.1结晶紫染色法.......................................................................................................16 5.1.1实验材料............................................................................................................16 5.1.2操作流程............................................................................................................17 全国团体标准信息平台 T/CI011—2021 I5.1.3观察与定量结果展示........................................................................................17 5.1.4技术要点............................................................................................................17 5.2死活菌细胞染色法...............................................................................................18 5.2.1实验材料............................................................................................................18 5.2.2操作流程............................................................................................................18 5.2.3观察与定量结果展示........................................................................................19 5.2.4技术要点............................................................................................................19 5.3CFU计数法..........................................................................................................19 5.3.1实验材料............................................................................................................20 5.3.2操作流程............................................................................................................20 5.3.3观察与定量结果展示........................................................................................21 5.3.4技术要点............................................................................................................21 5.4干重/湿重定量法..................................................................